Isolated Plin5-deficient cardiomyocytes store less lipid droplets than normal, but without increased sensitivity to hypoxia

Plin5 is abundantly expressed in the heart where it binds to lipid droplets (LDs) and facilitates physical interaction between LDs mitochondria. We isolated cardiomyocytes from adult Plin5+/+ Plin5-/- mice study role of for fatty acid uptake, LD accumulation, oxidation, tolerance hypoxia. Cardiomyocytes cultured with oleic stored less than Plin5+/+, but comparable levels when adipose triglyceride lipase activity was inhibited. The ability oxidize acids into CO2 similar cardiomyocytes, had a transient increase intracellular oxidation intermediates. After pre-incubation acids, retained higher content glycogen showed improved hypoxia compared Plin5+/+. In isolated, perfused hearts, deletion no important effect on ventricular pressures or infarct size after ischemia. Old reduced cardiac triacylglycerides, increased weight, apart modest elevated expression mRNAs beta myosin heavy chain Myh7 transporter Cd36, other genes involved metabolism glucose utilization were essentially unchanged by removal Plin5. seems facilitate storage primarily repressing without altering capacity. Expression has little importance acute ischemia, which contrasts protective mouse models during myocardial